| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5'-3' exoribonuclease 2; DHM1-like protein; DHP protein; XRN2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human XRN2 recombinant protein (Position: A5-N748). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects 5'-3' exoribonuclease 2 (gene XRN2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 108.6 kDa, and the supplier reports an observed band at about 109 kDa.
XRN2 is a nuclear 5′–3′ exoribonuclease with roles in RNA processing and degradation. After cleavage at the poly(A) site, it degrades the downstream RNA still attached to RNA polymerase II and helps release the polymerase, a model of transcription termination known as the torpedo mechanism; it also contributes to rRNA and other RNA processing.
| Target | 5'-3' exoribonuclease 2 (gene XRN2; UniProt Q9H0D6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 108.6 kDa |
| Observed MW | 109 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HEK293 cells, human PC-3 cells, human COLO-320 cells, human HepG2 cells, human THP-1 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human XRN2 fragment (Ala5–Asn748), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 5'-3' exoribonuclease 2 in the nucleus. Tissue expression noted by UniProt (human): Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
