| Field | Specification |
|---|---|
| Target | |
| Alternative names | dCTP pyrophosphatase 1; Deoxycytidine-triphosphatase 1; dCTPase 1; RS21C6; XTP3-transactivated gene A protein; DCTPP1; XTP3TPA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human XTP3TPA/DCTPP1 recombinant protein (Position: R22-T170). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects dCTP pyrophosphatase 1 (gene DCTPP1) in human and mouse samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 18.7 kDa, and the supplier reports an observed band at about 19 kDa.
DCTPP1 (dCTP pyrophosphatase 1) hydrolyzes deoxynucleoside triphosphates, especially dCTP, into their monophosphate forms. Its high activity toward modified dCTP analogs, such as 5-methyl-dCTP, suggests it helps keep damaged or non-canonical nucleotides out of DNA. It is studied in nucleotide pool sanitation, genome stability and cancer cell metabolism.
| Target | DCTP pyrophosphatase 1 (gene DCTPP1; UniProt Q9H773, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 18.7 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human Jurkat cells, human K562 cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human XTP3TPA/DCTPP1 fragment (Arg22–Thr170), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places dCTP pyrophosphatase 1 in the mitochondrion, nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
