| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nucleolysin TIA-1 isoform p40;RNA-binding protein TIA-1;T-cell-restricted intracellular antigen-1;TIA-1;p40-TIA-1;TIA1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Y14 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-RBM8A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22R85; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Y14 Rabbit Monoclonal Antibody catalog # M02769-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: RBM8A (Nucleolysin TIA-1 isoform p40).
- Antibody format: Monoclonal; clone 22R85; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
RBM8A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. Reported cellular localization context: Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage. Tissue expression notes (as provided): Widely expressed.
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,Apoptosis,Cancer,Cell Biology,Cell Death,DNA/RNA,Epigenetics and Nuclear Signaling,Immunology,Non-CD,Nucleus,RNA Processing,T Cells.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate RBM8A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RBM8A expression by Western blot in cell or tissue lysates, Detect RBM8A in FFPE tissue sections by immunohistochemistry, Localize RBM8A by immunofluorescence/immunocytochemistry in cultured cells, Quantify RBM8A-positive cells by flow cytometry in single-cell suspensions, Enrich RBM8A by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 19 kDa; calculated MW: 42963 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 19 kDa
- Cellular localization (provided): Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
- Tissue details (provided): Widely expressed.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
