| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcriptional coactivator YAP1;Yes-associated protein 1;Protein yorkie homolog;Yes-associated protein YAP65 homolog;YAP1;YAP65; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human YAP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CIE-25 is a rabbit monoclonal antibody that recognizes transcriptional coactivator YAP1 in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 54.5 kDa, and the supplier reports an observed band at about 70 kDa.
YAP1 is a transcriptional coactivator and the main downstream effector of the Hippo pathway. When Hippo signaling is active, LATS1/2 kinases phosphorylate YAP1 and keep it out of the nucleus; when the pathway is off, YAP1 enters the nucleus and partners with TEAD factors to drive genes for growth and survival, so it is widely studied in organ size control, mechanotransduction, regeneration and cancer.
| Target | Transcriptional coactivator YAP1 (gene YAP1; UniProt P46937, human) |
|---|---|
| Clone | CIE-25 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.5 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human uterus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human YAP1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcriptional coactivator YAP1 in the cytoplasm, nucleus, cell junction and cell membrane. Tissue expression noted by UniProt (human): Increased expression seen in some liver and prostate cancers. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hong-qiang Chen et al. (2024) KCNJ15 inhibits chemical-induced lung carcinogenesis and progression through GNB1 mediated Hippo pathway. Toxicology. 10.1016/j.tox.2024.154034
Jun Ge et al. (2019) PMMA Bone Cement Acts on the Hippo/YAP Pathway To Regulate CTGF and Induce Intervertebral Disc Degeneration. ACS Biomaterials Science & Engineering. 10.1021/acsbiomaterials.9b00146
Tang Xiaomin et al. (2019) Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathway. BMC Cancer. 10.1186/s12885-019-5832-9
