| Field | Specification |
|---|---|
| Target | |
| Alternative names | SRK; STD; TZK; ZAP70; ZAP-70; ZAP 70; P43403 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 9D5 is a mouse monoclonal antibody that recognizes tyrosine-protein kinase ZAP-70 (gene ZAP70) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 69.9 kDa, and the supplier reports an observed band at about 72 kDa.
ZAP-70 is a SYK-family tyrosine kinase that is recruited to phosphorylated ITAMs of the T cell receptor–CD3 complex after antigen recognition. Activated ZAP-70 phosphorylates adaptors such as LAT and SLP-76 to launch downstream T cell signaling, it is essential for thymocyte development, and it is studied in immunodeficiency and as a marker in chronic lymphocytic leukemia.
| Target | Tyrosine-protein kinase ZAP-70 (gene ZAP70; UniProt P43403, human) |
|---|---|
| Clone | 9D5 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 69.9 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human CCRF-CEM cells, rat thymus tissue, mouse spleen tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): 293T cells, Jurkat cells.
Immunogen
Synthetic peptide from the middle region of human ZAP70. It differs from the mouse sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase ZAP-70 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in T- and natural killer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
