| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger E-box-binding homeobox 1; NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8; ZEB1; AREB6; TCF8 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AREB6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADIO-26 is a rabbit monoclonal antibody that recognizes zinc finger E-box-binding homeobox 1 (gene ZEB1) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 124.1 kDa, and the supplier reports an observed band at about 200 kDa.
ZEB1 is a zinc finger E-box-binding homeobox transcription factor that represses epithelial genes such as E-cadherin, partly by recruiting the corepressor CtBP. It is a key driver of epithelial–mesenchymal transition (EMT) and is regulated by the miR-200 family, so it is widely studied in development, cancer invasion and metastasis.
| Target | Zinc finger E-box-binding homeobox 1 (gene ZEB1; UniProt P37275, human) |
|---|---|
| Clone | ADIO-26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 124.1 kDa |
| Observed MW | 200 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human AREB6.
Reactivity Notes
The supplier lists reactivity with human. UniProt places zinc finger E-box-binding homeobox 1 in the nucleus. Tissue expression noted by UniProt (human): Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Runsheng Guo et al. (2018) MiR-200a negatively regulates TGF-β1-induced epithelial-mesenchymal transition of peritoneal mesothelial cells by targeting ZEB1/2 expression. American Journal of Physiology-Renal Physiology. 10.1152/ajprenal.00566.2016
