Anti-Zebrafish Anp32e Antibody

SKU:BHA21011284
Research Validated
—
Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-anp32e antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Zebrafish. Recognizes acidic leucine-rich nuclear phosphoprotein 32 family member E. Supplied in liquid form, ready for use in standard immunodetection workflows.
Target anp32e
Host Rabbit
Reactivity Zebrafish
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
DZ41646 200 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 200 uL/vial
    • Conjugation (12): Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647, Carrier Free

    Variant-specific details:
    • 200 uL/vial + Unconjugated; 200 uL/vial + Carrier Free
      Storage: At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
      Form: Liquid
      Applications: IHC
      Application details: Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml
      Contents: Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
    • 200 uL/vial + Biotin; 200 uL/vial + Cy3; 200 uL/vial + Fluoro488; 200 uL/vial + Fluoro550; 200 uL/vial + Fluoro594; 200 uL/vial + FITC; 200 uL/vial + APC; 200 uL/vial + PE; 200 uL/vial + Fluoro647
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Form: Liquid
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 200 uL/vial + HRP
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Form: Liquid
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target anp32e
Alternative names Acidic leucine-rich nuclear phosphoprotein 32 family member E; anp32e
Gene ID 406562
UniProt # Q6NUW5
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Zebrafish
Applications
  • Immunohistochemistry
Conjugate
  • Biotin
  • Cy3
  • Fluoro488
  • Fluoro550
  • Fluoro594
  • FITC
  • HRP
  • APC
  • PE
  • Fluoro647
  • Carrier Free
Purification Immunogen affinity purified.
Cellular localization Cytoplasm. Nucleus.
Concentration 500 μg/ml
Form Liquid
Storage At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) DZ41646
Main SKU BHA21011284
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody targets acidic leucine-rich nuclear phosphoprotein 32 family member E (gene anp32e) in zebrafish samples and is validated for IHC.

In zebrafish, anp32e encodes a homolog of ANP32E, a histone chaperone that removes the histone variant H2A.Z from nucleosomes. By shaping where H2A.Z sits on chromatin, especially at enhancers, ANP32E contributes to chromatin organization and gene regulation.

Target Acidic leucine-rich nuclear phosphoprotein 32 family member E (gene anp32e; UniProt Q6NUW5, zebrafish)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Zebrafish
Form Liquid
Formulation 20 mM PBS; 50% glycerol; 0.02% NaN3
Storage Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–5 µg/mL

Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

Reactivity Notes

The supplier lists reactivity with zebrafish. UniProt places acidic leucine-rich nuclear phosphoprotein 32 family member E in the cytoplasm and nucleus.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 2–5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Zebrafish. Other species are not listed by the supplier.
Q.What are the host species and clonality?
A.Host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG.
Q.How should this antibody be stored?
A.Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Jia-xin Qiao et al. (2025) Multi-omics integration reveals that KLF improves stomach heat syndrome through intestinal flora remodeling and PI3K-Akt pathway activation. Fitoterapia. 10.1016/j.fitote.2025.107021

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