| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptotic protease-activating factor 1; APAF-1; apaf1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish APAF1 recombinant protein (Position: E414-I1247). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets apoptotic protease-activating factor 1 (gene apaf1) in zebrafish samples and is validated for IHC.
Apoptotic protease-activating factor 1 (APAF1) is the central scaffold of the apoptosome. When cytochrome c is released from mitochondria, APAF1 binds it and ATP/dATP, oligomerizes, and activates caspase-9, which then activates caspase-3 to execute the intrinsic pathway of apoptosis.
| Target | Apoptotic protease-activating factor 1 (gene apaf1; UniProt Q9I9H8, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish APAF1 fragment (Glu414–Ile1247), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places apoptotic protease-activating factor 1 in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
