| Field | Specification |
|---|---|
| Target | |
| Alternative names | Autophagy protein 5; atg5; apg5l; zgc:100934 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish ATG5 recombinant protein (Position: S118-L155). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets autophagy protein 5 (gene atg5) in zebrafish samples and is validated for IHC.
ATG5 is a core autophagy protein that is covalently linked to ATG12 by the enzymes ATG7 and ATG10. The ATG12-ATG5 conjugate, bound to ATG16L1, drives LC3 lipidation and autophagosome formation, and ATG5 knockout cells and animals are standard tools for studying autophagy.
| Target | Autophagy protein 5 (gene atg5; UniProt F1RDC0, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | Store at −20 °C for up to 12 months from receipt. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish cerebellum tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish ATG5 fragment (Ser118–Leu155), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places autophagy protein 5 in the cytoplasm and preautophagosomal structure membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
