| Field | Specification |
|---|---|
| Target | |
| Alternative names | Brain-derived neurotrophic factor; proBDNF; bdnf |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish BDNF recombinant protein (Position: A27-R278) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes brain-derived neurotrophic factor (gene bdnf) in zebrafish samples and is validated for Western blot and IHC.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin secreted as proBDNF and processed to mature BDNF, which signals mainly through the TrkB receptor (NTRK2). It supports survival and differentiation of neurons during development and regulates synaptic plasticity, learning and memory in the adult brain, making it a common target in neuroscience and psychiatry research.
| Target | Brain-derived neurotrophic factor (gene bdnf; UniProt A0A8M1P735, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): whole female zebrafish tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish spinal cord tissue, zebrafish eye tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish BDNF fragment (Ala27–Arg278), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt describes brain-derived neurotrophic factor as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
