| Field | Specification |
|---|---|
| Target | |
| Alternative names | Activator protein 1; AP 1; AP1; c Jun; JUN; jun oncogene; P39; Proto oncogene c Jun; Transcription factor AP 1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish c-Jun/JUN recombinant protein (Position: M1-F308). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets transcription factor Jun in zebrafish samples and is validated for Western blot and ICC/IF.
c-Jun (JUN) is a bZIP transcription factor and core component of AP-1. It forms dimers with Fos family proteins and other partners to bind TRE (TGA[GC]TCA) sites and control genes involved in proliferation, differentiation, stress responses and apoptosis. Its activity is strongly increased by JNK phosphorylation, and it is a widely used readout in studies of cell signaling and cancer; the zebrafish ortholog is also studied in development and regeneration.
| Target | Transcription factor Jun (gene jun; UniProt Q6NZT5, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (ICC/IF): zebrafish embryo tissue.
Immunogen
Recombinant zebrafish c-Jun/JUN fragment (Met1–Phe308), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places transcription factor Jun in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
