| Field | Specification |
|---|---|
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein CASC3 in zebrafish samples and is validated for Western blot and IHC.
In zebrafish, casc3 encodes a homolog of CASC3 (also called MLN51 or Barentsz), a component of the exon junction complex (EJC) that is deposited on mRNAs during splicing. The EJC influences mRNA export, localization, translation and nonsense-mediated decay, and it is studied in RNA processing and development.
| Target | Protein CASC3 (gene casc3; UniProt Q1ECZ4, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Liquid |
| Formulation | 20 mM PBS; 50% glycerol; 0.02% NaN3 |
| Observed MW | 76 kDa |
| Storage | Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
Samples with a confirmed band (WB): zebrafish, zebrafish head tissueIHC: zebrafish brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places protein CASC3 in the cytoplasm, nucleus, nucleus speckle and cell projection. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
