| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-complex protein 1 subunit epsilon; CCT-epsilon; cct5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish CCT5 recombinant protein (Position: S442-D541). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets T-complex protein 1 subunit epsilon (gene cct5) in zebrafish samples and is validated for Western blot.
CCT5 is the epsilon subunit of the chaperonin TRiC (also called CCT), which folds actin, tubulin and numerous other cytosolic proteins in an ATP-dependent cycle. It is studied in protein quality control and cytoskeleton assembly, and CCT5 variants have been linked to a hereditary sensory neuropathy.
| Target | T-complex protein 1 subunit epsilon (gene cct5; UniProt Q6NVI6, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 59 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|
Samples with a confirmed band (WB): zebrafish head tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant zebrafish CCT5 fragment (Ser442–Asp541), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places T-complex protein 1 subunit epsilon in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
