Anti-Zebrafish COPE Antibody

SKU:BHA21011443
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-COPE antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Zebrafish. Recognizes coatomer subunit epsilon. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target COPE
Host Rabbit
Reactivity Zebrafish
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
AZQ5U3E8 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 ug/vial; Conjugation: Unconjugated / Size: 100 ug/vial; Conjugation: Carrier Free
      • Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
      • Form: Lyophilized
      • Applications: IHC
      • Application details: Immunohistochemistry, 2-5 μg/ml, Zebrafish
      • Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
    • Size: 100 ug/vial; Conjugation: Biotin / Size: 100 ug/vial; Conjugation: Cy3 / Size: 100 ug/vial; Conjugation: Fluoro488 / Size: 100 ug/vial; Conjugation: Fluoro550 / Size: 100 ug/vial; Conjugation: Fluoro594 / Size: 100 ug/vial; Conjugation: FITC / Size: 100 ug/vial; Conjugation: APC / Size: 100 ug/vial; Conjugation: PE / Size: 100 ug/vial; Conjugation: Fluoro647
      • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      • Form: Liquid
      • Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • Size: 100 ug/vial; Conjugation: HRP
      • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      • Form: Liquid
      • Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target COPE
Alternative names Coatomer subunit epsilon; cope; zgc:103652
Gene ID 492492
UniProt # Q5U3E8
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Zebrafish
Applications
  • Immunohistochemistry
Immunogen E.coli-derived zebrafish COPE recombinant protein (Position: E72-A300).
Purification Immunogen affinity purified.
Reconstitution Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Cellular localization Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) AZQ5U3E8
Main SKU BHA21011443
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody detects coatomer subunit epsilon (gene cope) in zebrafish samples and is validated for IHC.

COPE is the ε subunit of coatomer, the cytosolic complex that assembles COPI coats on Golgi membranes. COPI vesicles return proteins bearing dilysine motifs from the Golgi to the endoplasmic reticulum and move cargo between Golgi cisternae, so COPE is studied in secretory pathway and membrane trafficking research.

Target Coatomer subunit epsilon (gene cope; UniProt Q5U3E8, zebrafish)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Zebrafish
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–5 µg/mL (Zebrafish)

Samples with confirmed staining (IHC): zebrafish kidney tissue, zebrafish brain tissue, zebrafish colon tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Immunogen

Recombinant zebrafish COPE fragment (Glu72–Ala300), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with zebrafish. UniProt places coatomer subunit epsilon in the Golgi apparatus membrane.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 2–5 µg/mL (Zebrafish). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Zebrafish. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant zebrafish COPE fragment (Glu72–Ala300), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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