| Field | Specification |
|---|---|
| Target | |
| Alternative names | CCNB; CCNB1; cyclin B1; G2/mitotic specific cyclin B1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish Cyclin B1/CCNB1 recombinant protein (Position: M1-S398). |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects G2/mitotic-specific cyclin-B1 (gene ccnb1) in zebrafish samples and is validated for Western blot.
Cyclin B1 (CCNB1) binds and activates CDK1 to form maturation-promoting factor, the kinase that triggers entry into mitosis. Its levels rise through G2 and it is destroyed at the metaphase-to-anaphase transition, so it is a standard marker of G2/M cells in cell cycle and cancer studies.
| Target | G2/mitotic-specific cyclin-B1 (gene ccnb1; UniProt Q9IB44, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant zebrafish Cyclin B1/CCNB1 fragment (Met1–Ser398), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
