| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cholesterol side-chain cleavage enzyme, mitochondrial; Cholesterol desmolase; cyp11a1.1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish CYP11A1.1 recombinant protein (Position: K96-K487) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cholesterol side-chain cleavage enzyme, mitochondrial (gene cyp11a1.1) in zebrafish samples and is validated for Western blot.
In zebrafish, cyp11a1.1 encodes a mitochondrial cholesterol side-chain cleavage enzyme (P450scc), the first enzyme of steroid hormone synthesis. It converts cholesterol into pregnenolone and is expressed in steroidogenic tissues such as the interrenal gland and gonads, so it is used as a marker of steroidogenesis in zebrafish studies of development and endocrine function.
| Target | Cholesterol side-chain cleavage enzyme, mitochondrial (gene cyp11a1.1; UniProt Q7SYJ6, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant zebrafish CYP11A1.1 fragment (Lys96–Lys487), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places cholesterol side-chain cleavage enzyme, mitochondrial in the mitochondrion inner membrane and mitochondrion membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
