| Field | Specification |
|---|---|
| Target | |
| Alternative names | Keratin, type I cytoskeletal 18; Cytokeratin-18; CK-18; Dorsal aorta proneprin kinesin-1; DreK18; Keratin-18; K18; krt18; dapk1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish KRT18 recombinant protein (Position: R117-D416). |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes keratin, type I cytoskeletal 18 (gene krt18) in zebrafish samples and is validated for Western blot and IHC.
Keratin 18 (KRT18) is a type I intermediate filament protein of simple (single-layer) epithelia, such as those of the liver, intestine and glands. With its partner keratin 8 it forms filament networks that protect cells from mechanical and other stresses. Caspase-cleaved KRT18 fragments are used as markers of epithelial apoptosis, and KRT18 is also studied in zebrafish epithelia.
| Target | Keratin, type I cytoskeletal 18 (gene krt18; UniProt Q7ZTS4, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish eye tissue, zebrafish kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish KRT18 fragment (Arg117–Asp416), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
