| Field | Specification |
|---|---|
| Target | |
| Alternative names | Doublesex- and mab-3-related transcription factor 1; dmrt1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish DMRT1 recombinant protein (Position: R44-E76). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects doublesex- and mab-3-related transcription factor 1 (gene dmrt1) in zebrafish samples and is validated for IHC.
DMRT1 is a transcription factor with a DM DNA-binding domain that is central to male sex determination and testis development. It maintains Sertoli cell identity and controls the switch between mitosis and meiosis in spermatogonia. DMRT1 is conserved across vertebrates and is studied in sex differentiation, spermatogenesis and germ cell tumors.
| Target | Doublesex- and mab-3-related transcription factor 1 (gene dmrt1; UniProt Q71MM5, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish ovary tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish DMRT1 fragment (Arg44–Glu76), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places doublesex- and mab-3-related transcription factor 1 in the nucleus. Tissue expression noted by UniProt (zebrafish): Expressed in developing germ cells of both testis and ovary.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
