| Field | Specification |
|---|---|
| Target | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes dual specificity protein phosphatase (gene dusp4) in zebrafish samples and is validated for IHC.
In zebrafish, dusp4 encodes a dual-specificity protein phosphatase of the MAP kinase phosphatase family. Members of this family remove phosphate from threonine and tyrosine residues of MAP kinases to limit their activity; the specific roles of zebrafish dusp4 are less well characterized.
| Target | Dual specificity protein phosphatase (gene dusp4; UniProt Q7SZF3, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Liquid |
| Formulation | 20 mM PBS; 50% glycerol; 0.02% NaN3 |
| Storage | Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL |
|---|
Samples with confirmed staining (IHC): zebrafish heart tissue, zebrafish kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places dual specificity protein phosphatase in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
M. Fethullah Simsek et al. (2024) The vertebrate segmentation clock drives segmentation by stabilizing Dusp phosphatases in zebrafish. Developmental Cell. 10.1016/j.devcel.2024.11.003
