| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cadherin-1; E-cadherin; cdh1; E-cad |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish E-cadherin/CDH1 recombinant protein (Position: Y23-D864) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cadherin-1 (gene cdh1) in zebrafish samples and is validated for Western blot and IHC.
E-cadherin (CDH1) is the calcium-dependent adhesion protein that builds adherens junctions between epithelial cells. Its cytoplasmic tail links to the actin cytoskeleton through catenins, and loss of E-cadherin is a hallmark of epithelial–mesenchymal transition and is well documented in diffuse gastric and lobular breast cancers.
| Target | Cadherin-1 (gene cdh1; UniProt Q90Z37, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish inner ear tissue, zebrafish colon tissue, zebrafish esophageal epithelium tissue, zebrafish heart tissue, zebrafish kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish E-cadherin/CDH1 fragment (Tyr23–Asp864), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places cadherin-1 in the cell junction, cell membrane, endosome and Golgi apparatus. Tissue expression noted by UniProt (zebrafish): Expressed in the skin and the ovary (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
