| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 4E-1A; eIF4E-1A; mRNA cap-binding protein; eif4ea |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects eukaryotic translation initiation factor 4E-1A (gene eif4ea) in zebrafish samples and is validated for Western blot, ICC/IF and immunoprecipitation.
In zebrafish, eif4ea encodes an eIF4E cap-binding protein. It recognizes the 7-methylguanosine cap of mRNAs and helps recruit ribosomes during translation initiation, and it may also support nuclear export of certain mRNAs.
| Target | Eukaryotic translation initiation factor 4E-1A (gene eif4ea; UniProt Q9DFS6, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Liquid |
| Formulation | 20 mM PBS; 50% glycerol; 0.02% NaN3 |
| Storage | Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
| Immunoprecipitation | 2–4 µg/mL |
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places eukaryotic translation initiation factor 4E-1A in the cytoplasm and nucleus. Tissue expression noted by UniProt (zebrafish): Expressed in all tissues examined, including gill, fin, heart, intestine, muscle, ovary and testis.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
