| Field | Specification |
|---|---|
| Target | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish FHL2a/b recombinant protein (Position: K235-I279). |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects zgc:92428 (gene fhl2a) in zebrafish samples and is validated for IHC and ICC/IF.
In zebrafish, fhl2a is one of two paralogs of the four and a half LIM domains 2 gene. Its specific function has not been fully characterized, but the related mammalian protein FHL2 is a LIM-domain adaptor involved in muscle and heart biology.
| Target | Zgc:92428 (gene fhl2a; UniProt Q6AZB7, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Zebrafish) |
Samples with confirmed staining (ICC/IF): zebrafish embryo tissue.
Samples with confirmed staining (IHC): zebrafish kidney tissue, zebrafish brain tissue, zebrafish colon tissue, zebrafish heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish FHL2a/b fragment (Lys235–Ile279), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places zgc:92428 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
