| Field | Specification |
|---|---|
| Target | |
| Alternative names | 17 beta HSD 1; 20 alpha HSD; E17KSR; E2DH; EDH17B1; EDH17B2; EDHB17; HSD17; HSD17B1; SDR28C1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish HSD17B1 recombinant protein (Position: E99-K293). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes estradiol 17-beta-dehydrogenase (gene hsd17b1) in zebrafish samples and is validated for IHC.
17β-hydroxysteroid dehydrogenase type 1 (HSD17B1) converts the weak estrogen estrone into the potent estrogen estradiol, using NADPH. It is highly expressed in placenta and ovarian granulosa cells, and it is studied in estrogen-dependent tissues and hormone-sensitive cancers.
| Target | Estradiol 17-beta-dehydrogenase (gene hsd17b1; UniProt F1QUP2, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish ovary tissue, zebrafish brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish HSD17B1 fragment (Glu99–Lys293), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places estradiol 17-beta-dehydrogenase in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
