| Field | Specification |
|---|---|
| Target | |
| Alternative names | hsp90; hsp90a;hsp90aa1; fb17b01; hsp90a.1; zgc:86652; hsp90alpha; wu:fb17b01 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived Zebrafish HSP90A recombinant protein (Position: R393-G688). |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets heat shock protein HSP 90-alpha 1 (gene hsp90a.1) in zebrafish samples and is validated for Western blot, IHC and ICC/IF.
In zebrafish, hsp90a.1 encodes an inducible HSP90 alpha chaperone. Like mammalian HSP90, it uses ATP binding and hydrolysis to help fold and stabilize client proteins, and in zebrafish it is required in developing muscle for myosin folding and sarcomere assembly, making it a model for studying chaperones in muscle development.
| Target | Heat shock protein HSP 90-alpha 1 (gene hsp90a.1; UniProt Q90474, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): zebrafish muscle tissue, zebrafish brain tissue, zebrafish cardiac muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): zebrafish embryo tissue.
Immunogen
Recombinant zebrafish HSP90A fragment (Arg393–Gly688), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places heat shock protein HSP 90-alpha 1 in the melanosome and cytoplasm. Tissue expression noted by UniProt (zebrafish): Strongly expressed in the early embryos within the somitic slow muscle progenitors, the adaxial cells that lie on either side of the notochord but not the notochord. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
