Anti-Zebrafish IFT172 Antibody

SKU:BHA21011331
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-IFT172 antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Zebrafish. Recognizes intraflagellar transport protein 172 homolog. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target IFT172
Host Rabbit
Reactivity Zebrafish
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
AZQ5RHH4 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 ug/vial; Conjugation: Unconjugated / Size: 100 ug/vial; Conjugation: Carrier Free
      • Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
      • Form: Lyophilized
      • Applications: IHC
      • Application details: Immunohistochemistry, 2-5 μg/ml, Zebrafish
      • Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
    • Size: 100 ug/vial; Conjugation: Biotin / Size: 100 ug/vial; Conjugation: Cy3 / Size: 100 ug/vial; Conjugation: Fluoro488 / Size: 100 ug/vial; Conjugation: Fluoro550 / Size: 100 ug/vial; Conjugation: Fluoro594 / Size: 100 ug/vial; Conjugation: FITC / Size: 100 ug/vial; Conjugation: APC / Size: 100 ug/vial; Conjugation: PE / Size: 100 ug/vial; Conjugation: Fluoro647
      • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      • Form: Liquid
      • Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • Size: 100 ug/vial; Conjugation: HRP
      • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      • Form: Liquid
      • Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IFT172
Alternative names Intraflagellar transport protein 172 homolog; ift172
Gene ID 432389
UniProt # Q5RHH4
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Zebrafish
Applications
  • Immunohistochemistry
Immunogen E.coli-derived zebrafish IFT172 recombinant protein (Position: D1501-Q1732)
Purification Immunogen affinity purified.
Reconstitution Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Cellular localization Cell projection, cilium.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) AZQ5RHH4
Main SKU BHA21011331
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody recognizes intraflagellar transport protein 172 homolog (gene ift172) in zebrafish samples and is validated for IHC.

IFT172 is a large component of intraflagellar transport complex B, the machinery that carries cargo along cilia. It is needed to build and maintain cilia and is involved in the turnaround of IFT trains at the ciliary tip, and because Hedgehog signaling depends on primary cilia, it is also studied in ciliopathies and developmental signaling.

Target Intraflagellar transport protein 172 homolog (gene ift172; UniProt Q5RHH4, zebrafish)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Zebrafish
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–5 µg/mL (Zebrafish)

Samples with confirmed staining (IHC): zebrafish testis tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Immunogen

Recombinant zebrafish IFT172 fragment (Asp1501–Gln1732), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with zebrafish. UniProt places intraflagellar transport protein 172 homolog in the cell projection.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 2–5 µg/mL (Zebrafish). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Zebrafish. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant zebrafish IFT172 fragment (Asp1501–Gln1732), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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