| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-1; il1b; il1-b; zgc:111873 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish IL-1 beta/IL1B recombinant protein (Position: M1-I284) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interleukin-1 (gene il1b) in zebrafish samples and is validated for IHC.
Interleukin-1 beta (IL-1β) is a potent pro-inflammatory cytokine made as an inactive precursor and processed by caspase-1 after inflammasome activation. Acting through IL1R1 and IL1RAP, it induces fever, prostaglandin synthesis, neutrophil recruitment and Th17 differentiation, and it is one of the most studied mediators of inflammation.
| Target | Interleukin-1 (gene il1b; UniProt A0A8M1PI54, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish brain tissue, zebrafish cerebellum tissue, zebrafish kidney tissue, zebrafish colon tissue, zebrafish muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish IL-1 beta/IL1B fragment (Met1–Ile284), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt describes interleukin-1 as secreted and also located in the cytoplasm and lysosome.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
