| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrator complex subunit 12; Int12; ints12 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish INTS12 recombinant protein (Position: M1-K394) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets integrator complex subunit 12 (gene ints12) in zebrafish samples and is validated for Western blot, IHC and ICC/IF.
INTS12 is a subunit of the Integrator complex, which associates with RNA polymerase II and ends transcription close to gene promoters. Integrator also processes the 3′ ends of small nuclear RNAs, and INTS12 is studied as part of this machinery in transcriptional regulation.
| Target | Integrator complex subunit 12 (gene ints12; UniProt Q6IQU7, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish brain tissue, zebrafish spinal cord tissue, zebrafish kidney tissue, zebrafish eye tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): zebrafish embryo tissue.
Immunogen
Recombinant zebrafish INTS12 fragment (Met1–Lys394), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places integrator complex subunit 12 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
