| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kynurenine 3-monooxygenase; Kynurenine 3-hydroxylase; kmo |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes kynurenine 3-monooxygenase (gene kmo) in zebrafish samples and is validated for Western blot.
Kynurenine 3-monooxygenase (KMO) is an FAD-dependent enzyme of the outer mitochondrial membrane that converts L-kynurenine into 3-hydroxykynurenine in the kynurenine pathway of tryptophan breakdown. This step leads toward quinolinic acid, an NMDA receptor agonist, and NAD+ synthesis. KMO is studied in neurodegeneration, inflammation and pancreatitis, and in zebrafish it is also studied in development.
| Target | Kynurenine 3-monooxygenase (gene kmo; UniProt Q1RLY6, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Liquid |
| Formulation | 20 mM PBS; 50% glycerol; 0.02% NaN3 |
| Observed MW | 54 kDa |
| Storage | Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): zebrafish tissue, zebrafish head tissue, zebrafish body tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places kynurenine 3-monooxygenase in the mitochondrion outer membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
