| Field | Specification |
|---|---|
| Target | |
| Alternative names | N; 6; -adenosine-methyltransferase subunit METTL3; N(6)-adenosine-methyltransferase 70 kDa subunit; MT-A70; mettl3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish METTL3 recombinant protein (Position: D381-D575) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes N(6)-adenosine-methyltransferase subunit METTL3 in zebrafish samples and is validated for Western blot and IHC.
METTL3 is the catalytic subunit of the main m6A methyltransferase complex, which it forms with METTL14 and WTAP. It installs N6-methyladenosine, the most abundant internal modification of mRNA, thereby affecting mRNA splicing, export, stability and translation. METTL3 is widely studied in development, stem cell differentiation, immunity and cancer.
| Target | N(6)-adenosine-methyltransferase subunit METTL3 (gene mettl3; UniProt F1R777, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish METTL3 fragment (Asp381–Asp575), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places N(6)-adenosine-methyltransferase subunit METTL3 in the nucleus, nucleus speckle and cytoplasm. Tissue expression noted by UniProt (zebrafish): Expressed in the hemato-vascular system: enriched in sorted endothelial cells and haemogenic endothelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
