| Field | Specification |
|---|---|
| Target | |
| Alternative names | Otoferlin; Fer-1-like protein 2; otofa; fj34b10; otof; si:dkey-181f18.3; wu:fj34b10 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects otoferlin (gene otofa) in zebrafish samples and is validated for IHC.
In zebrafish, otofa is one of two otoferlin genes, which encode large calcium-sensing ferlin proteins in sensory hair cells. Zebrafish otoferlin is studied as a model for synaptic vesicle release in hair cells of the inner ear and lateral line.
| Target | Otoferlin (gene otofa; UniProt X1WB77, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Liquid |
| Formulation | 50% glycerol; 0.9% NaCl; 0.2% Na2HPO4; 0.02% NaN3 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL |
|---|
Samples with confirmed staining (IHC): zebrafish brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places otoferlin in the basolateral cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane and Golgi apparatus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
