| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-directed RNA polymerase II subunit RPB4; DNA-directed RNA polymerase II subunit D; polr2d; rnap; wu:fa95b11; zgc:110435 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish POLR2D recombinant protein (Position: A10-D127). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA-directed RNA polymerase II subunit RPB4 (gene polr2d) in zebrafish samples and is validated for IHC.
POLR2D (RPB4) is a subunit of RNA polymerase II that pairs with RPB7 to form a stalk-like subcomplex. This subcomplex helps keep the polymerase clamp closed during transcription and can bind single-stranded DNA and RNA, and POLR2D is studied as part of the core transcription machinery.
| Target | DNA-directed RNA polymerase II subunit RPB4 (gene polr2d; UniProt A0A8M1N062, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|
Samples with confirmed staining (IHC): zebrafish brain tissue, zebrafish spinal tissue, zebrafish muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish POLR2D fragment (Ala10–Asp127), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places DNA-directed RNA polymerase II subunit RPB4 in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
