| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rab3 GTPase-activating protein catalytic subunit; rab3gap1; rab3gap |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish RAB3GAP1 recombinant protein (Position: N109-Q798) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects rab3 GTPase-activating protein catalytic subunit (gene rab3gap1) in zebrafish samples and is validated for Western blot and IHC.
RAB3GAP1 is the catalytic subunit of the Rab3 GTPase-activating complex, which switches off Rab3 proteins by accelerating GTP hydrolysis. Together with RAB3GAP2 it also activates Rab18, and RAB3GAP1 mutations cause Warburg micro syndrome, which affects eye, brain and genital development; zebrafish rab3gap1 is also studied.
| Target | Rab3 GTPase-activating protein catalytic subunit (gene rab3gap1; UniProt Q6NUV0, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish brain tissue, zebrafish kidney tissue, zebrafish colon tissue, zebrafish heart tissue, zebrafish liver tissue, zebrafish spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish RAB3GAP1 fragment (Asn109–Gln798), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places rab3 GTPase-activating protein catalytic subunit in the cytoplasm, endoplasmic reticulum and Golgi apparatus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
