| Field | Specification |
|---|---|
| Target | |
| Alternative names | Splicing factor 3A subunit 1; sf3a1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish SF3A1 recombinant protein (Position: K16-P646). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes splicing factor 3A subunit 1 (gene sf3a1) in zebrafish samples and is validated for IHC and ICC/IF.
Splicing factor 3A subunit 1 (SF3A1, SF3a120) is a component of the SF3a complex within the U2 snRNP of the spliceosome. SF3a helps the U2 snRNP engage the intron branch site and select the branch-point adenosine during early spliceosome assembly, so SF3A1 is studied in pre-mRNA splicing.
| Target | Splicing factor 3A subunit 1 (gene sf3a1; UniProt Q90X41, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Zebrafish) |
Samples with confirmed staining (IHC): zebrafish spinal cord tissue, zebrafish eye tissue, zebrafish muscle tissue, zebrafish kidney tissue, zebrafish colon tissue, zebrafish brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): zebrafish embryo tissue.
Immunogen
Recombinant zebrafish SF3A1 fragment (Lys16–Pro646), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places splicing factor 3A subunit 1 in the nucleus speckle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
