| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-box transcription factor TBX5-A; zftbx5a; T-box protein 5; zTbx5; tbx5a; heartstrings; hst; tbx5; tbx5.1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish TBX5A recombinant protein (Position: M1-S492) |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects T-box transcription factor TBX5-A (gene tbx5a) in zebrafish samples and is validated for Western blot.
In zebrafish, tbx5a is one of two paralogs of the T-box transcription factor TBX5. It is required for pectoral fin formation and, together with tbx5b, for heart looping and eye development, making zebrafish a useful model for studying TBX5 in heart and limb development.
| Target | T-box transcription factor TBX5-A (gene tbx5a; UniProt Q9IAK8, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant zebrafish TBX5A fragment (Met1–Ser492), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places T-box transcription factor TBX5-A in the nucleus and cytoplasm. Tissue expression noted by UniProt (zebrafish): Expressed in the dorsal optic cup of developing eye, pectoral fin buds and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
