| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor AP-2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2; tfap2a; ap2; ap2a; cb93; fb83f04; fc31a07; wu:fb83f04; wu:fc31a07 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets transcription factor AP-2-alpha (gene tfap2a) in zebrafish samples and is validated for IHC and ICC/IF.
AP-2α (TFAP2A) is a transcription factor that binds GC-rich AP-2 sites as a dimer. It is particularly important in neural crest cells and for development of the face, eye, limbs and body wall, and TFAP2A mutations cause branchio-oculo-facial syndrome.
| Target | Transcription factor AP-2-alpha (gene tfap2a; UniProt A0A2R8QRQ5, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Liquid |
| Formulation | 20 mM PBS; 50% glycerol; 0.02% NaN3 |
| Storage | Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 4 µg/mL |
Samples with confirmed staining (IHC): zebrafish esophagus tissue, zebrafish brain tissue, zebrafish kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): zebrafish embryos tissue.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places transcription factor AP-2-alpha in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
