| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transitional endoplasmic reticulum ATPase; TER ATPase; Protein CDC48; Valosin-containing protein; VCP; cdc48 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived zebrafish VCP recombinant protein (Position: D10-K512). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes transitional endoplasmic reticulum ATPase (gene vcp) in zebrafish samples and is validated for Western blot and IHC.
VCP (p97) is an abundant AAA+ ATPase that uses ATP to extract ubiquitinated proteins from membranes, chromatin and protein complexes. With cofactors such as UFD1 and NPL4 it drives ER-associated degradation and supports autophagy, DNA repair and Golgi reassembly. VCP mutations cause multisystem proteinopathy, and it is studied in protein homeostasis and neurodegeneration.
| Target | Transitional endoplasmic reticulum ATPase (gene vcp; UniProt Q7ZU99, zebrafish) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Zebrafish |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Zebrafish) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Zebrafish) |
Samples with a confirmed band (WB): zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): zebrafish spinal cord tissue, zebrafish brain tissue, zebrafish colon tissue, zebrafish liver tissue, zebrafish eye tissue, zebrafish muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant zebrafish VCP fragment (Asp10–Lys512), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with zebrafish. UniProt places transitional endoplasmic reticulum ATPase in the cytoplasm, endoplasmic reticulum and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
