| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse ZIP Kinase/Dapk3 recombinant protein (Position: R290-R448). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ZIP Kinase/Dapk3 Antibody Picoband® is an antibody for Dapk3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Dapk3 (glutathione S-transferase mu 3); UniProt: O54784
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 53 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-ZIP Kinase/Dapk3 Antibody Picoband® catalog # A03300-3.
Biological background
Biological context: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.
Expression and localization notes: cellular localization: Cytoplasm., tissue context: Testis and brain..
Common research applications
- Western blotting (WB): Compare Dapk3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Dapk3 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify Dapk3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Death-associated protein kinase 3 is an enzyme that in humans is encoded by the DAPK3 gene. Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm.
- Tissue details: Testis and brain.
- Research category: Cancer,Drug Metabolism,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
