Anti-ZNF609 Antibody Picoband®

Overview

Anti-ZNF609 Antibody Picoband® is an antibody for ZNF609 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: ZNF609 (kelch repeat and BTB domain containing 2); UniProt: O15014
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 190 kDa, calculated 24145 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-ZNF609 Antibody Picoband® catalog # A15674-1.

Biological background

Biological context: Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Expression and localization notes: cellular localization: Cul3-RING ubiquitin ligase complex., tissue context: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon..

Common research applications

• Western blotting (WB): Compare ZNF609 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of ZNF609 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify ZNF609-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. zinc finger protein 609 (ZNF609) is a 1,411 amino acid member of the Krüppel C2H2-type zinc-finger rotein family. Localized to the nucleus, ZNF609 contains one C2H2-type zinc finger through which it is thought to be involved in DNA-binding and transcriptional regulation.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cul3-RING ubiquitin ligase complex.
• Tissue details: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
• Research category: Cell Biology

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.