APRT polyclonal antibody

SKU:BHA11307713
Overview
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Anti-APRT antibody (Rabbit). Validated for Western Blot, Immunohistochemistry, Immunofluorescence. Reactive against Human, Mouse, Rat targets.
Host Species Rabbit
Species Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Validated Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Method Affinity Purification
Options selector
Catalog no. Size
BS7588-50UL 50 uL
BS7588-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL, 100 uL
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No BS7588
Alternative Names Adenine phosphoribosyltransferase; AMP; AMP diphosphorylase; AMP pyrophosphorylase; APRT; APT_HUMAN; DKFZp686D13177; MGC125856; MGC125857; MGC129961; Transphosphoribosidase;
Conjugate
  • Unconjugated
Formulation Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Host Rabbit
Immunogen Recombinant full length Human APRT.
Molecular Weight ~ 19 kDa
Product Type
  • Primary Antibody
Purity > 95% (by SDS-PAGE)
Reactivity
  • Human
  • Mouse
  • Rat
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product Overview

Research Use Only

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP).

Validated Applications

Western Blot (WB) 500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Immunohistochemistry (IHC) 50 - 1:200 IF 1:50 - 1:200
Immunofluorescence (IF/ICC) See datasheet for recommended dilution

Key Specifications

Host Species Rabbit
Species Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Purification Affinity Purification
Formulation Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Molecular Weight ~ 19 kDa
Catalog No. BS7588
UniProt P07741
Alternative Names Adenine phosphoribosyltransferase; AMP; AMP diphosphorylase; AMP pyrophosphorylase; APRT; APT_HUMAN; DKFZp686D13177; MGC125856; MGC125857; MGC129961; Transphosphoribosidase;

Immunogen / Antigen

Recombinant full length Human APRT.

Reactivity Notes

APRT polyclonal antibody detects endogenous levels of APRT protein.

Storage & Handling

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the APRT polyclonal antibody been validated for?

This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Recombinant full length Human APRT.

How should this antibody be stored and handled?

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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