| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C23H21N3O3 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
APS-2-79 functions as a KSR-dependent antagonist of MEK, blocking ATP-biotin binding to KSR2 within the KSR2-MEK1 complex with an IC50 of 120 nM. By stabilizing the inactive state of KSR, it antagonizes oncogenic Ras-MAPK signaling[1]. It is supplied as a white to off-white solid (C23H21N3O3, MW 387.43) at 99.38% purity.
Physical & Chemical Properties
| CAS Number | 2002381-25-9 |
|---|---|
| Molecular Formula | C23H21N3O3 |
| Molecular Weight | 387.43 g/mol |
| Purity | 99.38% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | CC1=CC(OC2=CC=CC=C2)=CC=C1NC3=NC=NC4=CC(OC)=C(OC)C=C43 |
| Target | KSR2, MEK1 |
| Signaling Pathway | MAPK/ERK Pathway |
| Solubility | In Vitro: DMSO: 20 mg/mL (51.62 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 2 years; -20°C, 1 year. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target[1]
|
KSR2 120 nM (IC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 20 mg/mL (51.62 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 2 years) or -20°C (up to 1 year); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2 mg/mL (5.16 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (20.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | ≥ 2 mg/mL (5.16 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (20.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2 mg/mL (5.16 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (20.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
At 5 μM, APS-2-79 suppresses KSR-stimulated MEK and ERK phosphorylation in 293H cells[1]. At 1 μM, APS-2-79 boosts the efficacy of the clinical MEK inhibitor trametinib in cancer cell lines carrying K-Ras mutations[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Cell Assay[1]
Run cell viability assays in 96 well plates. Determine optimal cell densities for the 96 well plate assays so that growth is linear over the time course of the assays. Plate A549, HCT-116, A375, SK-MEL-239, COLO-205, LOVO, SK-MEL-2, CALU-6, MEWO, SW620 and SW1417 cells at 500 cells per well and treat with inhibitors (e.g., APS-2-79; 100-3,000 nM) for 72hrs before measuring viability. Plate H2087 and HEPG2 cells at 2000 cells per well and treat with inhibitors (e.g., APS-2-79; 100-3,000 nM) for 72hrs. Measure cell viability with Resazurin, and determine the percent cell viability by normalizing inhibitor-treated samples to DMSO controls[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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