| Field | Specification |
|---|---|
| Alternative names | ARF5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Form | Liquid |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a matched capture and detection antibody pair designed for sandwich ELISA quantification of ADP-ribosylation factor 5 in Human,Rat,Guinea pig,Horse,Pig,Dog,Goat,Sheep. The pair consists of a capture antibody (unconjugated) and a detection antibody (biotin-conjugated), both raised in Capture: Rabbit | Detection: Rabbit, enabling a complete sandwich format without the need for additional antibody sourcing.
Key elements and design rationale
- Clonality: Capture: Polyclonal | Detection: Polyclonal — each component is independently validated for capture and detection roles in the sandwich format.
- Conjugation format: Capture: Unconjugated | Detection: Biotin conjugated — the detection antibody carries the biotin label for streptavidin-amplified signal generation; the capture antibody is unconjugated for plate coating.
- Isotype: Capture: IgG | Detection: IgG — matching isotype across capture and detection components reduces non-specific background from secondary reagents.
- Application notes: We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
Biological background
ADP-ribosylation factor 5 (also referred to as ARF5) is a protein target of interest in Human,Rat,Guinea pig,Horse,Pig,Dog,Goat,Sheep systems. Researchers studying ADP-ribosylation factor 5 typically rely on quantitative sandwich ELISA to measure protein levels in serum, plasma, cell culture supernatants, or tissue homogenates. Consult UniProt, NCBI Gene, and primary literature for current annotation of ADP-ribosylation factor 5 expression, function, and disease relevance.
Common research applications
- Sandwich ELISA quantification: The matched pair is optimized for standard sandwich ELISA formats. The capture antibody is coated to the plate surface; after sample incubation, the biotinylated detection antibody is added, followed by streptavidin-HRP and colorimetric substrate for quantification.
- Sample types: Typically validated for serum, plasma, and cell culture supernatants. Confirm sample type compatibility with the product datasheet before use in other matrices.
Notes for experimental design
- Antibody concentrations: We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
- Standard curve: Use recombinant ADP-ribosylation factor 5 protein as a standard for absolute quantification; confirm standard purity and activity before use.
- Cross-reactivity: Species reactivity is reported for Human,Rat,Guinea pig,Horse,Pig,Dog,Goat,Sheep. Use in other species matrices requires empirical confirmation of antibody–antigen binding.
- Streptavidin-HRP: Select a high-quality streptavidin-HRP conjugate and optimize its concentration to minimize background while maintaining signal sensitivity.
This is a matched capture and detection antibody pair for sandwich ELISA quantification of ADP-ribosylation factor 5 in Human,Rat,Guinea pig,Horse,Pig,Dog,Goat,Sheep. The set includes one unconjugated capture antibody (for plate coating) and one biotin-conjugated detection antibody, both raised in Capture: Rabbit | Detection: Rabbit, allowing a complete sandwich ELISA without additional antibody sourcing.
Reported reactive against Human,Rat,Guinea pig,Horse,Pig,Dog,Goat,Sheep. Cross-reactivity with unlisted species should be confirmed empirically before use.
In a typical sandwich ELISA workflow: (1) coat the plate with the capture antibody (unconjugated) overnight; (2) block the plate and add samples containing ADP-ribosylation factor 5; (3) add the detection antibody (biotin-conjugated); (4) add streptavidin-HRP and a colorimetric substrate (e.g., TMB); (5) stop the reaction and read absorbance. We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher. These are recommended starting points; optimal concentrations should be determined experimentally by checkerboard titration varying both capture and detection antibody concentrations across your specific plate format and sample matrix.
Required additional reagents: (1) Streptavidin-HRP for biotin-detection signal generation; (2) Colorimetric substrate (e.g., TMB) and stop solution; (3) Standard curve — recombinant ADP-ribosylation factor 5 protein at known concentrations for absolute quantification; (4) Assay buffer and wash solution per your ELISA protocol. Confirm all reagents are compatible with your plate reader wavelength settings.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
