| Field | Specification |
|---|---|
| Target | |
| Alternative names | ARI3A_HUMAN; ARID domain containing 3A; ARID domain-containing protein 3A; ARID3A; AT rich interactive domain 3A (BRIGHT- like) protein; AT rich interactive domain 3A (BRIGHT-like); AT rich interactive domain-containing protein 3A; AT-rich interactive domain-containing protein 3A; B cell regulator of IgH transcription; B-cell regulator of IgH transcription; Bright; dead ringer like 1; dead ringer; Drosophila; homolog-like 1; Dead ringer-like protein 1; DRIL1; DRIL3; DRX; E2F binding protein 1; E2F-binding protein 1; E2FBP1; Homo sapiens AT rich interactive domain 3A (BRIGHT-like) |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human AT-rich interactive domain-containing protein 3A protein (26-137AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-ARID3A antibody raised in Rabbit, tested in ELISA, WB, IHC, IF. Designed to detect ARID3A in Human, Mouse for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Human AT-rich interactive domain-containing protein 3A protein (26-137AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
Biological background
ARID3A (also: ARI3A_HUMAN, ARID domain containing 3A, ARID domain-containing protein 3A, ARID3A, AT rich interactive domain 3A (BRIGHT- like) protein, AT rich interactive domain 3A (BRIGHT-like), AT rich interactive domain-containing protein 3A, AT-rich interactive domain-containing protein 3A, B cell regulator of IgH transcription, B-cell regulator of IgH transcription, Bright, dead ringer like 1, dead ringer, Drosophila, homolog-like 1, Dead ringer-like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2F-binding protein 1, E2FBP1, Homo sapiens AT rich interactive domain 3A (BRIGHT-like)) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ARID3A annotation relevant to epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
- WB: Confirms MW, distinguishes isoforms under denaturing conditions.
- IHC: Tissue expression in FFPE/frozen sections; optimize antigen retrieval.
- IF: Subcellular localization in fixed cells/sections; optimize fixation.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human, Mouse; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
ARID3A is a protein studied in Human, Mouse biology. This polyclonal antibody raised in Rabbit detects ARID3A in ELISA, WB, IHC, IF applications, IgG isotype.
Reported reactive against Human, Mouse. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA, WB, IHC, IF. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing ARID3A; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
