| Field | Specification |
|---|---|
| Target | |
| Alternative names | ascl1a; ash; ash1a; asha; piaAchaete-scute homolog 1a; Zash-1a; Pituitary-absent protein |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Zebrafish Achaete-scute homolog 1a protein (1-196AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-ascl1a antibody raised in Rabbit, tested in ELISA. Designed to detect ascl1a in Zebrafish for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Zebrafish Achaete-scute homolog 1a protein (1-196AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
Biological background
ascl1a (also: ascl1a, ash, ash1a, asha, piaAchaete-scute homolog 1a, Zash-1a, Pituitary-absent protein) — studied in Danio rerio (Zebrafish) (Brachydanio rerio) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ascl1a annotation relevant to epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Zebrafish; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
ascl1a is a protein studied in Zebrafish biology. This polyclonal antibody raised in Rabbit detects ascl1a in ELISA applications, IgG isotype.
Reported reactive against Zebrafish. Immunogen from Danio rerio (Zebrafish) (Brachydanio rerio). Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing ascl1a; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
