Asf1a Polyclonal Antibody

SKU:BHA12103309
Overview
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ASF1A-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunofluorescence, ELISA in Human, Mouse samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~23.0 kDa
UniProt Q9Y294
Gene ID Human: 25842; Mouse: 66403
Applications 3 validated
Options selector
Catalog no. Size
BT-AP00666-20UL 20 uL
BT-AP00666-50UL 50 uL
BT-AP00666-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP00666
Alternative Names ASF1A; CGI-98; HSPC146; Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 25842; Mouse: 66403
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human ASF1A. AA range:121-170
Isotype
  • IgG
Molecular Weight ~23.0 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
Storage -20°C for 1 year
Target ASF1A

Product Overview

Primary Antibody

This antibody targets ASF1A and detects endogenous levels of ASF1A protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunofluorescence (IF) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target ASF1A
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse
Purification Affinity Purification
Molecular Weight ~23.0 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human ASF1A. AA range:121-170
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 25842; Mouse: 66403
UniProt Q9Y294 (Human: Q9Y294; Mouse: Q9CQE6)

Scientific Background

ASF1A encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein (anti-silencing function 1A histone chaperone) is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.

Alternative Names

ASF1A; CGI-98; HSPC146; Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetASF1A
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse
PurificationAffinity Purification
Molecular Weight~23.0 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human ASF1A. AA range:121-170
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 25842; Mouse: 66403
UniProtQ9Y294 (Human: Q9Y294; Mouse: Q9CQE6)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunofluorescence (IF)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

ASF1A encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein (anti-silencing function 1A histone chaperone) is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.

Alternative Names

ASF1A; CGI-98; HSPC146; Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunofluorescence (IF), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of ASF1A?
The predicted molecular weight of ASF1A is ~23.0 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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