| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ATF2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding pro |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATF2. AA range:40-89 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
activating transcription factor 2(ATF2) Homo sapiens This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
| Immunoprecipitation | Refer to datasheet |
| ELISA | Refer to datasheet |
Immunogen & Specificity
Immunogen: The antiserum was produced against synthesized peptide derived from human ATF2. AA range:40-89
Subcellular Localization: Nucleus. Cytoplasm. Mitochondrion outer membrane. Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).
UniProt/Swiss-Prot: P15336
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Observed Band (kDa) | 52kD |
| Storage | -20°C/1 year |
Reactivity Notes
Confirmed reactivity in Human, Mouse, Rat samples; validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for ATF-2 in the BioHippo catalog.
This antibody has been validated for Western Blot, Immunohistochemistry, Immunofluorescence. Refer to the product datasheet for recommended dilutions and protocol guidance for each application.
Refer to the datasheet for recommended dilution ranges per application. Optimal dilution may vary by sample type, fixation method, and detection system — a titration experiment is recommended prior to full experimental use.
This antibody has been validated or predicted to recognize Human, Mouse, Rat samples, based on immunogen sequence homology. For unlisted species, contact our team for guidance.
This antibody was raised against a specific peptide or recombinant protein fragment corresponding to ATF-2. Exact immunogen details are provided in the product datasheet.
Conjugated formats may be available upon request. Contact the BioHippo team for custom conjugation options including fluorophore, biotin, or enzyme labeling.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
