| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP synthase subunit alpha, mitochondrial|ATP synthase F1 subunit alpha|ATP5F1A|ATP5A|ATP5A1|ATP5AL2|ATPM |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes ATP5F1A. It is validated for ELISA, Western Blot, Immunohistochemistry.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:500 - 1:2000 |
| IHC | 1:50 - 1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Immunogen | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 54 kDa |
| UniProt # | P25705 |
| Gene ID | 498 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Alternative Names | ATP synthase subunit alpha, mitochondrial|ATP synthase F1 subunit alpha|ATP5F1A|ATP5A|ATP5A1|ATP5AL2|ATPM |
Reactivity Notes
Validated reactivity: Human, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
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Explore related antibodies and reagents for ATP5F1A research from Fine Test, or contact us for alternative formats.
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