| Field | Specification |
|---|---|
| Target | |
| Alternative names | Atp5f1b; Atp5b; ATP synthase subunit beta; mitochondrial; EC 7.1.2.2; ATP synthase F1 subunit beta |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Mouse ATP synthase subunit beta, mitochondrial protein (47-529AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-Atp5f1b antibody raised in Rabbit, conjugated with FITC. Designed to detect Atp5f1b in Mouse, supporting researchers in tags & cell markers contexts.
Key elements and design rationale
- Immunogen: Recombinant Mouse ATP synthase subunit beta, mitochondrial protein (47-529AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
- FITC conjugate: Direct fluorescence (Ex ~490 nm / Em ~525 nm). Susceptible to photobleaching — use antifade reagents; protect from light.
Biological background
Atp5f1b (also known as Atp5f1b, Atp5b, ATP synthase subunit beta, mitochondrial, EC 7.1.2.2, ATP synthase F1 subunit beta) is a protein studied in Mus musculus (Mouse) systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of Atp5f1b biology relevant to tags & cell markers.
Common research applications
- Refer to the product datasheet for confirmed application data.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Mouse; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
Atp5f1b is a protein target in Mouse biology. This polyclonal antibody raised in Rabbit detects Atp5f1b and is validated for immunoassay applications.
This antibody is reported reactive against Mouse. Immunogen derived from Mus musculus (Mouse). Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Application data not specified — review the product datasheet or contact our technical team.
FITC (Ex ~490 nm / Em ~525 nm) enables direct fluorescence without secondary. Protect from light; use antifade mounting for slides. FITC is susceptible to photobleaching.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing Atp5f1b; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
