AtT-20 cell

The AtT-20 cell line is a well-characterized mouse pituitary tumor cell line derived from anterior pituitary cells. These cells originate from a strain of mice known as AtT-20/D16v-F2, and are primarily used for the study of pituitary function and regulation, especially focusing on the synthesis and secretion of adrenocorticotropic hormone (ACTH). ACTH is crucial for adrenal gland function and is a key player in the stress response and metabolic regulation. AtT-20 cells exhibit typical features significant for studies in neuroendocrinology and pharmacology, such as the production and secretion of pro-opiomelanocortin (POMC), the precursor molecule for ACTH. The cells are responsive to corticotropin-releasing hormone (CRH) and other hypothalamic hormones, making them an excellent model for exploring the hypothalamic-pituitary-adrenal (HPA) axis in vitro. Moreover, AtT-20 cells can be used to investigate the mechanisms of peptide hormone processing, packaging, and secretion, given their well-defined secretory pathways. In terms of applications, AtT-20 cells have been utilized in various studies including those focusing on gene expression profiles under different treatment conditions, intracellular signaling pathways involving cAMP, and the effects of genetic modifications on hormone secretion. These cells are also valuable in the assessment of the pharmacological properties of potential drug candidates targeting HPA axis components.

SKU:BHC11101161

• SDS (10A38169B85D411B8D4353C302F06D34)
• Product Sheet (10A38169B85D411B8D4353C302F06D34)
• AtT-20 305161-300824 Cytion CoA (PDF)
• AtT-20 305161-011024 Cytion CoA (PDF)

Protein expression: Adrenocorticotropic Hormone(Acth)

• cultureMedium: Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3 (Cytion article number 820608a)
• supplements: Supplement the medium with 2.5% FBS, 15% horse serum
• dissociationReagent: Accutase
• subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth.
• fluidRenewal: 2 to 3 times per week
• freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.