| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C24H26N4O2 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
AZ PFKFB3 26 is a potent, selective PFKFB3 inhibitor with an IC50 of 23 nM, and also inhibits PFKFB1 and PFKFB2 with IC50 values of 2.06 μM and 0.384 μM, respectively. It can be used to study non-small cell lung cancer[1]. It is supplied as a light yellow to khaki solid (C24H26N4O2, MW 402.49) at 99.23% purity.
Physical & Chemical Properties
| CAS Number | 1704740-52-2 |
|---|---|
| Molecular Formula | C24H26N4O2 |
| Molecular Weight | 402.49 g/mol |
| Purity | 99.23% |
| Appearance | Solid |
| Color | Light yellow to khaki |
| SMILES | O=C([C@H]1NCCC1)NC2=CC=C(OC3=CC4=C(N(CC(C)C)C=C4C#N)C=C3)C=C2 |
| Signaling Pathway | Autophagy |
| Solubility | In Vitro: DMSO: 5 mg/mL (12.42 mM; Requires sonication and adjust pH to 3 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 2 years; -20°C, 1 year. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
IC50: 23 nM (PFKFB3), 2.06 μM (PFKFB1), 0.384 μM (PFKFB2)[1]
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 5 mg/mL (12.42 mM) | requires sonication and adjust pH to 3 with HCl; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 2 years) or -20°C (up to 1 year); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
PFKFB3 is potently inhibited by AZ PFKFB3 26 (IC50 of 23 nM)[1]. In A549 cells, AZ PFKFB3 26 downregulates F-1,6-BP levels, with an IC50 of 343 nM[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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PFKFB3 activates CAD to enhance de novo pyrimidine synthesis for cell growth. Cell Rep 2025 Jul 29;44(8):116071. PMID: 40742808
Simultaneous inhibition of PFKFB3 and GLS1 selectively kills KRAS-transformed pancreatic cells. Biochem Biophys Res Commun 2021 Sep 24:571:118-124. PMID: 34325126
