| Field | Specification |
|---|---|
| Cellosaurus | CVCL_YD53 |
| Species | |
| Applications | |
| Classification | |
| Tissue | |
| Disease | Carcinoma |
| Age | |
| Sex | |
| Ethnicity | |
| Growth properties | |
| Biosafety level | |
| Catalog no. (Mfr.) | |
| Main SKU |
- Surface antigens: CD19
- Viruses: Transformant: EBV
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% heat-inactivated FBS
- subculturing: Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 105 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.